<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE html PUBLIC "-//ABISOURCE//DTD XHTML plus AWML 2.2//EN" "http://www.abisource.com/2004/xhtml-awml/xhtml-awml.mod">
<html xmlns="http://www.w3.org/1999/xhtml" xmlns:awml="http://www.abisource.com/2004/xhtml-awml/">
 <head>
  <!-- ======================================================= -->
  <!-- Created by AbiWord, a free, Open Source wordprocessor.  -->
  <!-- For more information visit http://www.abisource.com.    -->
  <!-- ======================================================= -->
  <meta http-equiv="content-type" content="text/html;charset=UTF-8" />
  <title>manual.htm</title>
  <meta name="Title" content="manual.htm" />
  <style type="text/css">
   <!--
#toc,
.toc,
.mw-warning {
	border: 1px solid #aaa;
	background-color: #f9f9f9;
	padding: 5px;
	font-size: 95%;
}
#toc h2,
.toc h2 {
	display: inline;
	border: none;
	padding: 0;
	font-size: 100%;
	font-weight: bold;
}
#toc #toctitle,
.toc #toctitle,
#toc .toctitle,
.toc .toctitle {
	text-align: center;
}
#toc ul,
.toc ul {
	list-style-type: none;
	list-style-image: none;
	margin-left: 0;
	padding-left: 0;
	text-align: left;
}
#toc ul ul,
.toc ul ul {
	margin: 0 0 0 2em;
}
#toc .toctoggle,
.toc .toctoggle {
	font-size: 94%;
}@media print, projection, embossed {
	body {
		padding-top:1in;
		padding-bottom:1in;
		padding-left:1in;
		padding-right:1in;
	}
}
body {
	font-family:'Times New Roman';
	font-variant:normal;
	text-indent:0in;
	widows:2;
	font-style:normal;
	font-weight:normal;
	text-decoration:none;
	color:#000000;
	text-align:left;
	font-size:12pt;
}
table {
}
td {
	border-collapse:collapse;
	text-align:left;
	vertical-align:top;
}
p, h1, h2, h3, li {
	color:#000000;
	font-family:'Times New Roman';
	font-size:12pt;
	text-align:left;
	vertical-align:normal;
}
*.block_text {
	margin-bottom:6pt;
	margin-left:1in;
	margin-right:1in;
}
     -->
  </style>
 </head>
 <body>
  <div>
   <p></p>
   <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in"><span style="font-size:16pt">Multiphoton Guide</span></p>
   <p class="numbered_list" awml:style="Numbered List"></p>
   <p><span style="font-weight:bold">Motion Control</span></p>
   <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in"></p>
   <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in"><span style="font-weight:bold">Keyboard</span></p>
   <p></p>
   <table cellpadding="0" border="0" style="width:100%;border:1pt;border-collapse:collapse;empty-cells:show;table-layout:fixed;border-style:solid">
    <colgroup>
     <col />
     <col />
    </colgroup>
    <tbody style="border:inherit">
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">x+</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">d</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">x-</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">a</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">y+</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">w</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">y-</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">s</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">z+</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">]</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">z-</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">[</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">theta+</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">=</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">theta-</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in">-</p>
      </td>
     </tr>
    </tbody>
   </table>
   <p style="text-align:left;margin-bottom:0.0000in;margin-top:0.0000in;margin-right:0.0000in"></p>
   <p>Position Readout</p>
   <p>Objective Angle</p>
   <p>Rotation Mode</p>
   <p>Absolute Mode</p>
   <p>Relative Mode</p>
   <p>Set Relative Origin</p>
   <p>Coarse/Fine Knob</p>
   <p></p>
   <p></p>
   <p><span style="font-weight:bold">Image Acquisition</span></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Disable Scanner - Activate first to start resonant scanning. 5 minutes warmup before imaging is ideal.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Monitor - Opens shutter and starts acquisition for monitoring. </p>
   <p></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Acquire - Opens shutter and begins acquisition of raw data. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Save monitor - Allows saving of monitor image sequence to disk (TIFF 16-bit</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Ext trigger - Begins monitor or acquire on TLL input to Trigger in</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Retriggerable - In combination with Ext trigger, allows trigger re-arm after monitor or acquire finishes</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Stop trigger - In combination with Ext trigger, allows second TTL on Trigger in to stop acquisition.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Dry run acquire - Allows preview of raw 8-bit data during acquire. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Sum during acquire - Allows summation preview during acquire. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Fragment files - Breaks TIFF into smaller TIFFs during save monitor or acquire.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Path - Path for TIFF storage.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Dataset - Name for TIFF storage.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Filename - Displays serialized filename for acquire or save monitor.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Frames - Number of frames to acquire or save monitor before stop.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Fragment size - In combination with Fragment files, allows TIFFs of finite size to be stored for a single acquire or save monitor.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Acquisition No. - Displays the current acquisition number appended to TIFF filenames. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"><span style="font-weight:bold;font-size:12pt">Scanning</span></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p>Lines/frame - Lines scanned by resonant scanner per frame. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Magnification - Fast way to change fast axis and slow axis amplitude in a manner which maintains 1:1 pixel aspect ratio.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Fast axis scan - Change fast axis (resonant) scanner amplitude from 0.0 to 1.0. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Slow axis scan - Change slow axis scanner amplitude from 0.0 to 1.0. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Res. delay - Resonant scanning sync pulse delay adjustment (hardware). </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Save delay - Force save of Res. delay tuning.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Slow flyback - Fraction of slow (vertical) scan to be used for flyback. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Laser power - Controls pockels cell modulation from 0.0 - 1.0 (0.0V - 2.0V resp). </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Slow axis blank - Use pockels cell to blank laser during slow flyback.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Fast axis blank - Use pockels cell to blank laser at edges of resonant scan.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Lock tune - Lock tuning of slow/fast blanking.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Slow blank tune (Delay) - Adjust phase of slow flyback blanking, 0.00-1.00.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Slow blank tune (Width) - Adjust duration of slow flyback blanking, as a fraction of flyback command duration.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Fast blank tune (Freq) - Fast blanking requires a buffered voltage output once per frame, and requires a one-time adjustment to match (and hence measure) the true resonant scanner frequency (approximately 7.95kHz). </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Fast blank tune (Delay) - After fast blank tune frequency is found, pulse delay can be fine tuned to match the horizontal scan edges. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p><span style="font-weight:bold">Monitor Post-processing</span></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Median - Performs 3x3 median filter on summed/averaged monitor image.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Mean - Performs 3x3 mean filter.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Log - Takes logarithm of image.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Mirror X - Flips x dimension.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Mirror Y - Flips y dimension.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Sine - Carries out nearest neighbor inverse sine mapping to compensate for sinusoidal fast scan trajectory.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">AC coupling - Changes from AC coupling to DC coupling of input video signal.</p>
   <p></p>
   <p>Sum Frames - Number of frames for temporal summation, during monitor. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Reset Display - Re-initializes acquisition card.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Lock ADC Min Max - Locks Ch1-3 Adjustment tabs from accidental adjustment.</p>
   <p><span style="font-weight:bold"><br />Overlay</span></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Show overlay - Shows display image annotation fields, to save information directly over image data.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Frame number - Shows frame number in overlay.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Position - Shows position in overlay.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Crosshairs - Shows center cross hairs in overlay.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Scalebars - Shows (uncalibrated) scale bars in overlay.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Show exposure - Shows framerate or sum frames number in overlay.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Show ROIs - Shows 8 ROIs used in analysis window for raw trace plotting.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Save Current Display to Overlay - Saves current Multiphoton Display image, and copies to overlay buffer.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Show overlay image - Shows overlay buffer image in overlay.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p><span style="font-weight:bold">Analysis</span></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Show analysis window - Shows window for ROI raw trace plotting (one trace per color per ROI), and for histogram plotting.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Compute ROI traces - Computes ROI raw traces during monitor, requiring more CPU usage.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Compute Histograms - Computes histogram of raw images (0-255), to verify dynamic range or over-range before raw acquisition. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p><span style="font-weight:bold">Ch 1-3</span></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Black and White - Adjust linear LUT for display of monitor images.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">ADC Max (V) - Adjust hardware ADC max reference voltage, ie this signal level corresponds to 255 in raw data.</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">ADC Min (V) - Adjust hardware ADC min reference voltage, ie this signal level corresponds to 0 in raw data. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Monochrome - View this channel as monochrome image in display. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">Save Channel - Determines whether this channel will be saved to TIFF during raw acquisition. </p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p>Digital Ocular Port</p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in"></p>
   <p class="numbered_list" awml:style="Numbered List" style="margin-left:0pt;text-indent:0in">PMT/Camera - Select Utility DB9 pin 1/2 voltage orientation, to actuate between PMT path and camera path. </p>
   <p></p>
   <p><span style="font-weight:bold">Scripting Function</span></p>
   <p></p>
   <p style="text-align:left">By creating a text file (using MATLAB, etc) in the program directory titled "multiphoton_script.txt,</p>
   <p style="text-align:left"></p>
   <p style="text-align:left">A queue of commands is stored, and they are sequentially executed. </p>
   <p style="text-align:left"></p>
   <p style="text-align:left">Each command takes the form of series of characters, followed by three floating point parameters, space or tab separated.</p>
   <p style="text-align:left"></p>
   <p style="text-align:left">Example:</p>
   <p style="text-align:left"></p>
   <blockquote><span style="font-family:'Courier New'">move 100.0 -100.0 0.0</span></blockquote>
   <blockquote><span style="font-family:'Courier New'">changelaserpower</span></blockquote>
   <blockquote><span style="font-family:'Courier New'">savemonitor 0.0 0.0 0.0</span></blockquote>
   <p style="text-align:left"></p>
   <p style="text-align:left">This script moves the microscope +100 microns in X, -100 microns in Y, and 0 microns in Z. </p>
   <p style="text-align:left"></p>
   <p style="text-align:left"></p>
   <table cellpadding="0" border="0" style="width:100%;border:1pt;border-collapse:collapse;empty-cells:show;table-layout:fixed;border-style:solid">
    <colgroup>
     <col />
     <col />
     <col />
     <col />
     <col />
    </colgroup>
    <tbody style="border:inherit">
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p>Command</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Command</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Parameter</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Parameter B</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Parameter C</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Move the microscope incrementally (delta) in microns</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>moverel</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>dX move (microns)</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>dY move (microns)</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>dZ move (microns)</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Adjust laser power</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>changelaserpower</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>laser power (V)</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Initiate monitoring</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>monitor</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Initiate acquisition</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>aquire</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Initiate fast-stack</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>faststack</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Change length of acquisition (number of frames)</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left">numberframes</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>number of frames</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>Add a integer prefix to dataset name, to achieve customized filename</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left">datasetprefix</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>prefix (e.g. 1253.0 adds 1253_ to the dataset name)</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>external trigger checkbox</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left">externaltriggeron</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>external triggercheckbox</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left">externaltriggeroff</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>retriggercheckbox</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left">retriggeron</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>retrigger checkbox</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left">retriggeroff</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>save monitorcheckbox</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left">savemonitoron</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>save monitorcheckbox</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p style="text-align:left">savemonitoroff</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>&nbsp;</p>
       <p></p>
       <p></p>
       <p>theta move (radians)</p>
       <p></p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p>theta</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p>angle of move, in radians</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p>theta&nbsp;radius move (radians), virtual gimbal</p>
       <p></p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p>thetaradius</p>
       <p></p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p>angle of move, in radians</p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p>unused</p>
       <p></p>
       <p></p>
      </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p>unused</p>
      </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
     </tr>
     <tr style="border:inherit">
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
      <td style="padding: 0.00pt;border:inherit inherit inherit">
       <p></p>
       <p></p>
       <p></p>
       </td>
     </tr>
    </tbody>
   </table>
   <p style="text-align:left"></p>
   <p style="text-align:left"></p>
   <p style="text-align:left"></p>
   <p></p>
   <p></p>
   <p></p>
   <p></p>
   <p></p>
  </div>
  <div>
   <p></p>
  </div>
 </body>
</html>
